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Updated: Aug 9, 2026
Establishment of an Oronasal Fistula Mice Model
Published on: September 8, 2023
Development of a murine model of chronic rhinosinusitis
Robin Lindsay1, Tiffani Slaughter, Joy Britton-Webb
1National Naval Medical Center, and the Uniformed Services University of the Health Sciences, Bethesda, Maryland 20889-5612, USA. rwlindsay@bethesda.med.navy.mil
Objective:
The aim of this study was to develop a mouse model of chronic eosinophilic rhinosinusitis.
Study Design:
Mice were sensitized to Aspergillis fumigatus (Af) extract by intraperitoneal injection. The animals subsequently received nasal challenges with Af extract 3 times per week for 12 weeks. Sinonasal complexes were studied histologically by the study otolaryngologists and pathologists to characterize the inflammatory response.
Setting:
Animal care facility at an academic institution.
Results:
A chronic eosinophilic inflammatory response was evoked in all study animals. Statistical analysis was performed for inflammation, secretory cell hyperplasia, mast cells, and eosinophils. There were very significant differences (P<0.0005) between control and study mice in all categories.
Conclusion:
Prolonged nasal challenge of Af extract creates an inflammatory response in murine nasal mucosa that mimics human chronic eosinophilic rhinosinusitis.
Significance:
A murine model for chronic rhinosinusitis is reported that may facilitate future investigations into disease pathophysiology.
Ebm Rating:
B-2.
