Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genetic interactions between TFIIF and TFIIS.

Rachel N Fish1, Michelle L Ammerman, Judith K Davie

  • 1Department of Molecular and Cell Biology, University of California, Berkeley 94720-3202, USA.

Genetics
|May 2, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Oxidative stress as a pathway linking maternal phthalate and bisphenol exposure to childhood allergy and lung function: Mediation and interaction analyses.

Environmental research·2026
Same author

Epidemiology of child disadvantage and developmental vulnerability in Australia: Insights from linked administrative data.

Public health·2025
Same author

Evaluating analytic strategies to obtain high-resolution, vertex-level measures of cortical neuroanatomy in children in low- and middle-income countries.

Communications biology·2025
Same author

Beyond the silver bullet: closing the equity gap for children within a generation.

The Medical journal of Australia·2024
Same author

Association of Maternal Air Pollution Exposure and Infant Lung Function Is Modified by Genetic Propensity to Oxidative Stress.

Children (Basel, Switzerland)·2024
Same author

Cord blood lipid correlation network profiles are associated with subsequent attention-deficit/hyperactivity disorder and autism spectrum disorder symptoms at 2 years: a prospective birth cohort study.

EBioMedicine·2024
Same journal

Coexistence of piRNA and KZFP defense systems: Evolutionary dynamics of layered defense against transposable elements.

Genetics·2026
Same journal

Creation and manipulation of bipartite expression transgenes in C. elegans using phiC31 recombinase.

Genetics·2026
Same journal

Inherited long telomeres induce a genome-wide transcriptional response in budding yeast.

Genetics·2026
Same journal

Adaptive Dynamics of Quantitative Traits in a Steadily Changing Environment.

Genetics·2026
Same journal

Functional Landscape of Zebrafish Gonadotropins and Receptors: A Comprehensive Genetic Analysis.

Genetics·2026
Same journal

Synergistic actions of Nup43 and Myosin VI drive actin cone assembly during Drosophila spermiogenesis.

Genetics·2026
See all related articles

The eukaryotic transcript elongation factor TFIIS (PPR2) interacts genetically with TFIIF subunits (TFG1, TFG2). TFIIS

Area of Science:

  • Molecular Biology
  • Yeast Genetics

Background:

  • TFIIS (PPR2) is a eukaryotic transcript elongation factor.
  • TFIIS is essential when TAF14/TFG3 is absent.
  • Taf14p is part of the TFIIF complex, which also includes TFG1 and TFG2.

Purpose of the Study:

  • Investigate the synthetic lethality between ppr2Delta and taf14Delta.
  • Identify Taf14p-containing complexes involved in this lethality.
  • Explore functional connections between TFIIS and TFIIF.

Main Methods:

  • Genetic interaction analysis in Saccharomyces cerevisiae.
  • Construction and analysis of deletion and chimeric PPR2 gene constructs.
  • Assessment of cell growth under various genetic and temperature conditions.

Related Experiment Videos

Main Results:

  • Synthetic lethality observed between ppr2Delta and taf14Delta.
  • Mutant alleles of tfg1 or tfg2 showed improved growth at low temperatures without TFIIS.
  • The N-terminal 130 amino acids of TFIIS were crucial for complementing lethality in taf14Delta ppr2Delta cells.
  • The N-terminal region of TFIIS mediated deleterious effects on tfg1 or tfg2 cold-sensitive cells.

Conclusions:

  • TFIIS and TFIIF exhibit a physiologically relevant functional connection.
  • The N-terminal domain of TFIIS mediates an undefined function critical in the absence of Taf14p.
  • TFIIS and Taf14p are not functionally redundant, but their absence creates specific dependencies.