Characterization of an activation factor released from human neutrophils after stimulation by triclinic monosodium
Philippe Desaulniers1, Sébastien Marois, Guillaume Paré
1Centre de recherche en rhumatologie-immunologie du CHUL, Department of Medicine, Faculty of Medicine, Universite Laval, Quebec, Quebec, Canada.
Objective:
To determine the presence and characterize the activity of a soluble activation factor rapidly released by human neutrophils after stimulation with monosodium urate (MSU) crystals.
Methods:
Supernatants from human neutrophils stimulated by MSU crystals for 5 to 60 min were tested for their ability to stimulate a chemotactic response, induce a mobilization of calcium, and increase the tyrosine phosphorylation levels in naive neutrophils.
Results:
Supernatant from neutrophils stimulated
Conclusion:
There is rapid liberation of a potent activation signal from neutrophils after interaction with MSU crystals. This activation factor can further stimulate surrounding neutrophils and contribute to amplification of the inflammatory response induced by MSU crystals.


