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Related Experiment Videos

Purification and Characterization of Wheat beta(2-->1) Fructan:Fructan Fructosyl Transferase Activity.

B R Jeong1, T L Housley

  • 1Agronomy Department, Purdue University, West Lafayette, Indiana 47907.

Plant Physiology
|September 1, 1992
PubMed
Summary

Researchers isolated fructan:fructan fructosyl transferase (FFT) activity in wheat, crucial for elongating fructan chains. This enzyme plays a key role in carbohydrate storage in wheat vegetative tissues.

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Area of Science:

  • Plant Biochemistry
  • Carbohydrate Metabolism
  • Enzymology

Background:

  • Fructans are primary storage carbohydrates in wheat vegetative tissues.
  • Fructan:fructan fructosyl transferase (FFT) is essential for fructan chain elongation.

Purpose of the Study:

  • To isolate and characterize fructan:fructan fructosyl transferase (FFT) activity in wheat.
  • To understand the role of FFT in wheat fructan synthesis.

Main Methods:

  • Wheat plants were subjected to cold treatment to induce fructan synthesis.
  • Fructosyl transferase activity was purified using salt precipitation and multiple chromatography techniques.
  • Enzyme activity was assessed for specificity, pH optimum, and temperature optimum.

Main Results:

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  • Purified FFT activity was distinct from other fructan-metabolizing enzymes.
  • The enzyme exhibited optimal activity at pH 6.5 and 30°C.
  • FFT specifically transferred fructosyl units from 1-kestose or other fructans to sucrose and other fructans, synthesizing inulin oligosaccharides.

Conclusions:

  • The isolated FFT activity is crucial for in vitro synthesis of fructan oligosaccharides in wheat.
  • This enzyme contributes significantly to the biosynthesis of fructans, the major storage carbohydrates in wheat.