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Updated: Aug 8, 2026

Generation of Marked and Markerless Mutants in Model Cyanobacterial Species
Published on: May 29, 2016
A Cyanobacterial Gene Encoding Peptidyl-Prolyl cis-trans Isomerase
M Hassidim1, R Schwarz, J Lieman-Hurwitz
1Department of Botany, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel.
Abstract:
The rotA gene encoding peptidyl-prolyl cis-trans isomerase has been identified, sequenced, and shown to be transcribed in the cyanobacterium Synechococcus sp. PCC7942. Inactivation of the gene by replacement of a region containing the open reading frame with a gene conferring kanamycin resistance resulted in merodiploids containing both the wild type and the modified genomic region. We were not able to isolate a kanamycin-resistant mutant in which all the genomic wild-type copies were substituted, which suggests that such replacement could have been lethal.
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