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[Tumor necrosis factor in medicinal interferon preparations].

V P Kuznetsov, A A Babaiants, I S Frolova

    Voprosy Virusologii
    |July 1, 1991
    PubMed
    Summary

    Virus induction stimulates leukocytes to produce tumor necrosis factor (TNF) and interferon-alpha (IFN-alpha). TNF production kinetics were rapid, independent of IFN priming, and unaffected by IFN antibodies.

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    [Diagnostics of ataxia-telangiectasia by the express-test found on the method of indirect immunofluorescence].

    Tsitologiia·2014

    Area of Science:

    • Immunology
    • Virology
    • Biochemistry

    Background:

    • Interferons (IFNs) are crucial cytokines in antiviral defense.
    • Tumor Necrosis Factor (TNF) is a pro-inflammatory cytokine with diverse biological activities.
    • The interplay between IFN and TNF production in response to viral stimuli requires further elucidation.

    Purpose of the Study:

    • To investigate the production of TNF by leukocytes stimulated with viruses in the presence of IFN-alpha.
    • To characterize the kinetics and regulatory mechanisms of TNF and IFN-alpha biosynthesis.
    • To determine the presence of TNF in various IFN preparations.

    Main Methods:

    • Leukocyte cultures were induced with viruses in the presence of IFN-alpha.
    • Kinetics of TNF and IFN-alpha production were monitored over time.

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  • Antibodies against IFN-alpha and TNF were used to assess their roles in biosynthesis.
  • Commercial IFN preparations (native, recombinant, and chemically purified) were analyzed for TNF content.

    • Main Results:

    • Virus induction of leukocyte cultures with IFN-alpha resulted in rapid TNF production within 7-8 hours.
    • TNF and IFN-alpha biosynthesis kinetics were independent of the type of IFN used for priming.
    • Antibodies to IFN-alpha did not inhibit TNF production, and anti-TNF antibodies did not reduce IFN-alpha yields.
    • Detectable TNF was found in native IFN-alpha preparations but absent in recombinant and chemically purified IFN preparations.

    Conclusions:

    • Leukocytes stimulated by viruses in conjunction with IFN-alpha efficiently produce TNF.
    • TNF and IFN-alpha biosynthesis are distinct processes with rapid kinetics, not significantly influenced by each other's presence or antibodies.
    • The presence of TNF in native IFN preparations highlights potential variability and warrants consideration in therapeutic applications.