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Glycolate pathway in green algae.

W J Bruin1, E B Nelson, N E Tolbert

  • 1Department of Biochemistry, Michigan State University, East Lansing, Michigan 48823.

Plant Physiology
|September 1, 1970
PubMed
Summary
This summary is machine-generated.

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The glycolate metabolic pathway is confirmed in unicellular green algae through assimilation and enzyme presence. Algal glycolate metabolism differs from higher plants, with slower glycine-serine interconversion and additional rapid glycolate/glycine metabolism pathways.

Area of Science:

  • Biochemistry
  • Plant Physiology
  • Microbiology

Background:

  • The glycolate pathway is crucial for photorespiration in plants.
  • Understanding this pathway in algae is key to comprehending carbon fixation and metabolism in diverse photosynthetic organisms.

Purpose of the Study:

  • To investigate the presence and characteristics of the glycolate metabolic pathway in unicellular green algae.
  • To compare the glycolate pathway's operation in algae with that of higher plants.

Main Methods:

  • Assimilation of exogenous glycolate-1-(14)C and photosynthetic (14)CO(2) fixation experiments.
  • Assay of key glycolate pathway enzymes: P-glycolate phosphatase, glycolate dehydrogenase, l-glutamate:glyoxylate aminotransferase, serine hydroxymethylase, and glycerate dehydrogenase.
  • Analysis of (14)C labeling patterns in glycolate, glycine, and serine under different conditions, including the presence of isonicotinylhydrazide.

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Main Results:

  • Unicellular green algae assimilate and metabolize exogenous glycolate to glycine and serine.
  • Five key enzymes of the glycolate pathway were detected in algae grown on air.
  • Labeling patterns during (14)CO(2) fixation showed differences from higher plants, indicating slower glycine-serine interconversion and rapid metabolism of glycolate and glycine.
  • Glycolate dehydrogenase and serine hydroxymethylase were identified as potential rate-limiting enzymes.

Conclusions:

  • The glycolate pathway is demonstrably present and functional in unicellular green algae.
  • Significant differences exist in the regulation and kinetics of the glycolate pathway between algae and higher plants.
  • Algae exhibit rapid metabolism of glycolate and glycine, suggesting additional metabolic routes beyond the canonical glycolate pathway.