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The climacteric in ripening tomato fruit.

D J Chalmers1, K S Rowan

  • 1Botany School, University of Melbourne, Parkville, Victoria, 3052, Australia.

Plant Physiology
|September 1, 1971
PubMed
Summary

Orthophosphate activates phosphofructokinase, a key enzyme in tomato fruit respiration. This finding explains the climacteric rise in respiration during fruit ripening, impacting tomato quality.

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Area of Science:

  • Plant Physiology
  • Biochemistry
  • Fruit Ripening

Background:

  • The climacteric rise in respiration is a critical stage in fruit ripening.
  • Phosphofructokinase (PFK) is a key regulatory enzyme in glycolysis.
  • The specific triggers for PFK activation during tomato fruit ripening remain unclear.

Purpose of the Study:

  • To identify the regulatory enzyme responsible for the climacteric rise in tomato fruit respiration.
  • To investigate the role of orthophosphate as an activator of phosphofructokinase during fruit ripening.

Main Methods:

  • Infiltration of tomato fruit with orthophosphate and measurement of respiration rates.
  • Culturing tomato plants at varying phosphorus nutrient levels.
  • Correlation analysis between fruit orthophosphate concentration and respiration rates.

Main Results:

  • Phosphofructokinase was identified as the key regulatory enzyme activated at the onset of the climacteric rise.
  • Infiltration with orthophosphate led to a sustained increase in respiration rate.
  • Fruit respiration rates at the climacteric stage correlated with orthophosphate concentration.

Conclusions:

  • Orthophosphate is proposed as the primary activator of phosphofructokinase in ripening tomato fruit.
  • Increased cytoplasmic orthophosphate concentration stimulates phosphofructokinase, contributing to the climacteric rise in respiration.
  • This mechanism provides insight into the regulation of fruit ripening and respiration.

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