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Ultrastructural transformations in bean inner mitochondrial membranes.

R H Wilson1, E L Thurston, R Mitchell

  • 1Department of Botany, University of Texas, Austin, Texas 78712.

Plant Physiology
|January 1, 1973
PubMed
Summary
This summary is machine-generated.

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Bean mitochondria exhibit ultrastructural changes linked to respiration. Unlike mammalian mitochondria, bean mitochondria do not reverse configurations, indicating unique osmotic regulation in plant cells.

Area of Science:

  • Plant Cell Biology
  • Mitochondrial Physiology
  • Biochemistry

Background:

  • Mitochondria undergo configurational changes (condensed-to-orthodox) related to metabolic state, observed in mammalian systems.
  • Understanding plant mitochondrial ultrastructure and its dynamic changes is crucial for comprehending plant energy metabolism.

Purpose of the Study:

  • To investigate the ultrastructure of bean (Phaseolus vulgaris) shoot mitochondria in various metabolic states.
  • To compare plant mitochondrial configurational changes with those reported in mammalian mitochondria.
  • To correlate ultrastructural changes with osmotic volume and ion transport.

Main Methods:

  • Isolation of inner membrane-matrix mitochondria from bean shoots.
  • Transmission electron microscopy (TEM) to observe ultrastructure.

Related Experiment Videos

  • Spectrophotometric analysis of light absorbancy to monitor mitochondrial volume changes.
  • Measurement of potassium (K+) and inorganic phosphate (P(i)) uptake.
  • Assessment of respiratory activity.

    • Main Results:

    • Bean mitochondria displayed ultrastructural transformations analogous to mammalian condensed-to-orthodox changes upon transition from nonrespiring to respiring metabolism.
    • Mitochondria remained in the orthodox state with induced oxidative phosphorylation; the reverse orthodox-to-condensed transition was not observed.
    • Substrate and P(i) induced swelling in bean mitochondria, correlated with K+ and P(i) uptake and a slight respiratory stimulation.
    • Swollen mitochondria did not change volume upon initiation of phosphorylation, indicating a link between configuration and osmotic state.

    Conclusions:

    • Plant mitochondria undergo metabolic-dependent ultrastructural configuration changes similar to mammalian mitochondria.
    • Bean mitochondria lack the reverse orthodox-to-condensed transition, suggesting distinct regulatory mechanisms.
    • Configurational changes in bean mitochondria reflect internal osmotic adjustments, correlating with volume and ion flux.