Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Phytochrome properties and the molecular environment.

R E Kendrick1, C J Spruit

  • 1Laboratory of Plant Physiological Research, Agricultural University, Wageningen, The Netherlands.

Plant Physiology
|October 1, 1973
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Photocontrol of anthocyanin biosynthesis in tomato.

Journal of plant research·2016
Same author

Kinetics of phytochrome decay in Amaranthus seedlings.

Planta·2014
Same author

Photocontrol of germination in Amaranthus caudatus.

Planta·2014
Same author

The in vivo properties of Amaranthus phytochrome.

Planta·2014
Same author

Phytochrome in seeds of Amaranthus caudatus.

Planta·2014
Same author

Phytochrome in cucumber seeds.

Planta·2014

Phytochrome

Area of Science:

  • Plant biology
  • Photochemistry
  • Molecular spectroscopy

Background:

  • Phytochrome phototransformation is crucial for plant development.
  • In vivo and in vitro studies have yielded paradoxical results regarding phytochrome behavior.
  • Understanding phytochrome's molecular environment is key to resolving these paradoxes.

Purpose of the Study:

  • To elucidate the in vitro mechanisms of phytochrome phototransformation.
  • To investigate the influence of the molecular environment on phytochrome intermediates and dark reversion.
  • To reconcile in vitro findings with in vivo observations of phytochrome's physiological roles.

Main Methods:

  • In vitro spectrophotometric analysis of phytochrome phototransformation.
  • Investigation of phytochrome cycling and dark reversion kinetics.

Related Experiment Videos

  • Comparative analysis of in vitro data with in vivo physiological responses.

    • Main Results:

    • A sequential pathway for phytochrome phototransformation involving intermediates was supported in vitro.
    • The molecular environment significantly impacts phytochrome intermediate fractions and dark reversion rates.
    • Inverse dark reversion (Pr to Pfr) was observed in vitro.
    • In vitro findings suggest that localized phytochrome populations may control specific physiological responses.

    Conclusions:

    • Phytochrome's molecular environment dictates its transformation and reversion kinetics.
    • Discrepancies between in vivo and in vitro phytochrome studies can be explained by cellular localization.
    • Phytochrome's specific localization within the cell may determine its physiological activity.