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Updated: Aug 8, 2026

An Easy Method for Plant Polysome Profiling
Published on: August 28, 2016
Distribution of ribosomal deoxyribonucleic Acid in subcellular fractions of higher plants
1Department of Botany, University of Georgia, Athens, Georgia 30602.
Abstract:
Methods are described by which ribosomal DNA can be enriched in subcellular fractions of carrot and soybean. With both carrot and cucumber it was possible to obtain a distinct satellite DNA which contained the rDNA. Hybridization values greater than 0.49% were necessary before a satellite component was observed. Saturation hybridization values for soybean, carrot, and cucumber DNA were 0.2, 0.49, and 1.14%, respectively. These values were increased 1.6- and 2-fold in soybean and carrot, respectively, but enrichment was not obtained for cucumber.
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