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Published on: May 21, 2019
Multiple amine oxidases in cucumber seedlings
1Department of Biological Sciences, University of California, Santa Barbara, California 93106.
Plant Physiology
|October 1, 1974
Summary
Cucumber seedlings contain three distinct amine oxidase enzymes. These enzymes, separated by ion exchange chromatography, show varied substrate and inhibitor sensitivities, potentially impacting indoleacetic acid biosynthesis.
Area of Science:
- Plant biochemistry
- Enzymology
- Plant physiology
Background:
- Amine oxidases play crucial roles in plant metabolism.
- Understanding these enzymes is key to elucidating metabolic pathways like indoleacetic acid biosynthesis.
Purpose of the Study:
- To investigate the amine oxidase activity in cucumber seedlings.
- To characterize and differentiate the various amine oxidases present.
- To explore the potential link between these enzymes and auxin biosynthesis.
Main Methods:
- Cell-free extracts from cucumber seedlings (Cucumis sativus L.) were prepared.
- Enzyme activity was assayed using tryptamine as a substrate.
- Ion exchange chromatography was employed to separate and purify the enzymes.
- Substrate specificity and inhibitor sensitivity of the isolated enzymes were determined.
Main Results:
- Cucumber seedling extracts exhibited significant amine oxidase activity.
- Three distinct amine oxidases were successfully separated via ion exchange chromatography.
- One enzyme showed characteristics of monoamine oxidase, while the other two resembled diamine oxidases.
- Enzymes displayed differential sensitivities to various amine oxidase inhibitors.
Conclusions:
- Cucumber seedlings possess a heterogeneous amine oxidase system.
- The characterized enzymes may be involved in the biosynthesis of indoleacetic acid.
- Further research is warranted to fully elucidate the role of these enzymes in plant growth and development.
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