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Updated: May 6, 2026

Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana

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Avocado fruit protoplasts: a cellular model system for ripening studies.

F W Percival¹, L G Cass, K R Bozak

¹Department of Biological Science, University of California, 93106, Santa Barbara, CA, USA.

Plant Cell Reports

|November 14, 2013

Summary

This summary is machine-generated.

Avocado fruit protoplasts retain ripening characteristics and can synthesize cellulase. These isolated cells are also effective for transient gene expression assays, demonstrating their utility in plant science research.

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Area of Science:

Plant Biochemistry
Molecular Biology
Fruit Ripening

Background:

Avocado (Persea americana cv. Hass) ripening involves complex biochemical changes.
Understanding these changes at the cellular level is crucial for agricultural applications.
Protoplasts offer a model system to study cellular processes during fruit development.

Purpose of the Study:

To investigate the biochemical characteristics of avocado mesocarp protoplasts during ripening.
To assess the utility of isolated protoplasts in transient gene expression studies.
To determine if protoplasts retain the ability to synthesize key ripening-related enzymes.

Main Methods:

Isolation of mesocarp protoplasts from avocado fruits at various ripening stages.
Radiolabeling of polypeptides to analyze protein synthesis patterns.
Detection of cellulase antigen in protoplasts and intact fruit tissue.
Electroporation of a chimeric gene (CaMV 35S promoter-chloramphenicol acetyltransferase) into protoplasts.

Main Results:

Protoplasts exhibited qualitative changes in radiolabel incorporation patterns correlating with fruit ripening stages.
Protoplasts synthesized cellulase (endo-ß-1,4-glucanase) antigen, mirroring intact propylene-treated fruit.
Isolated protoplasts from preclimacteric and climacteric fruit showed equal competence in transient gene expression assays.

Conclusions:

Avocado mesocarp protoplasts retain key biochemical characteristics of the parent tissue during ripening.
Isolated protoplasts serve as a viable system for studying fruit ripening and gene expression.
The findings support the use of protoplasts in future molecular and biochemical investigations of avocado fruit development.