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R E Christoffersen1, E Warm, G G Laties

  • 1Department of Biology, University of California, 90024, Los Angeles, CA, USA.

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Summary
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Avocado fruit ripening involves specific gene expression changes. Three messenger RNAs (mRNAs) significantly increase during ripening, suggesting a genetic link to this process.

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Area of Science:

  • Plant Biochemistry
  • Molecular Biology
  • Fruit Ripening

Background:

  • Fruit ripening is a complex developmental process.
  • Avocado (Persea americana Mill cv. Hass) ripening is characterized by a climacteric rise in respiration and ethylene production.

Purpose of the Study:

  • To investigate changes in messenger RNA (mRNA) populations during avocado fruit ripening.
  • To identify specific genes potentially involved in the avocado ripening process.

Main Methods:

  • Isolation of polyadenylated (poly(A)(+)) RNA from avocado fruit at four ripening stages.
  • In vitro translation of isolated mRNAs using a rabbit reticulocyte lysate system.
  • Two-dimensional gel electrophoresis of translation products to analyze protein expression.

Main Results:

  • Three specific mRNAs showed a dramatic increase in abundance during ripening.
  • The corresponding translation products had molecular weights of 80,000, 36,000, and 16,500 Daltons.
  • These changes correlated with the climacteric rise in respiration and ethylene production.

Conclusions:

  • Avocado fruit ripening is associated with the differential expression of specific genes.
  • The identified mRNAs likely play a role in the physiological and biochemical changes during ripening.