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Multiple forms of invertase in developing oat internodes.

R A Jones1, P B Kaufman

  • 1Department of Botany, University of Michigan, Ann Arbor, Michigan 48104.

Plant Physiology
|January 1, 1975
PubMed
Summary
This summary is machine-generated.

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Oat internode development involves three invertases. Soluble invertase I activity correlates with rapid growth, while soluble invertase II peaks later, and bound invertase activity changes with tissue maturation.

Area of Science:

  • Plant Biochemistry
  • Plant Physiology
  • Enzymology

Background:

  • Invertases are crucial enzymes in plant carbohydrate metabolism.
  • Understanding invertase activity during plant development is key to deciphering growth regulation.

Purpose of the Study:

  • To characterize the different invertase forms in developing oat internodes.
  • To investigate the temporal changes in invertase activity during oat growth.

Main Methods:

  • Separation of soluble invertases using ion-exchange chromatography (diethylaminoethylcellulose) and size-exclusion chromatography (Sephadex).
  • Analysis of kinetic constants, heat stability, and pH optima for each invertase isozyme.
  • Quantification of invertase activity at different developmental stages of oat internodes.

Related Experiment Videos

Main Results:

  • Three distinct invertase forms were identified: two soluble (I and II) and one bound (III).
  • Soluble invertase I activity peaked early, coinciding with rapid internode growth.
  • Soluble invertase II activity showed a later peak, and bound invertase activity varied with tissue maturation.

Conclusions:

  • Differential expression and activity patterns of oat invertases suggest distinct roles in internode development.
  • Invertase I is likely involved in early rapid growth, while invertase II may play a role in later developmental processes.
  • Bound invertase activity changes reflect its involvement in both growth and maturation phases.