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Updated: Aug 8, 2026

Polysome Purification from Soybean Symbiotic Nodules
Published on: July 1, 2022
Energy State and Dinitrogen Fixation in Soybean Nodules of Dark-grown Plants
T M Ching1, S Hedtke, S A Russell
1Department of Crop Science, Oregon State University, Corvallis, Oregon 97331.
Abstract:
Dark treatment of 25-day-old greenhouse-grown plants of inoculated soybean (Glycine max var. Chippewa) for 1 day reduced ATP by 70%, sucrose by 60%, total adenosine phosphates by 60%, ATP/ADP ratio by 55%, nitrogenase activity by 50%, and energy charge by 15% in nodules. The close correlation between nitrogenase activity and these energy parameters indicates that they may play a major role in regulating dinitrogen fixation in the symbiotic system.
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