Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structure of functional soybean sieve elements.

D B Fisher1

  • 1Department of Botany, University of Georgia, Athens, Georgia 30602.

Plant Physiology
|November 1, 1975
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Variations of the natural abundances of nitrogen and carbon isotopes in Triticum aestivum, with special reference to phloem and xylem exudates.

The New phytologist·2021
Same author

Cotton embryogenesis: The entrance and discharge of the pollen tube in the embryo sac.

Planta·2014
Same author

Cotton embryogenesis: The pollen cytoplasm.

Planta·2014
Same author

Cotton embryogenesis: The tissues of the stigma and style and their relation to the pollen tube.

Planta·2014
Same author

Cotton embryogenesis: The identification, as nuclei, of the X-bodies in the degenerated synergid.

Planta·2014
Same author

The estimation of sugar concentration in individual sieve-tube elements by negative staining.

Planta·2014
Same journal

TaSPL14-D diverged from its ortholog to regulate tiller angle in rice: a caveat for orthology-based functional inference.

Plant physiology·2026
Same journal

From wrinkled seeds to plant oil accumulation networks: The legacy of a Plant Physiology classic.

Plant physiology·2026
Same journal

LcHXK1 mediates glucose signaling to inhibit fruit abscission by phosphorylating LcWRKY42, a feedback regulator in lignin polymerization.

Plant physiology·2026
Same journal

Partial submergence-induced adventitious root emergence in cucumber requires CsRBOHB-mediated ROS production.

Plant physiology·2026
Same journal

JA differentially regulates a SmWLIM1/MYB62-SUS1 module to control male fertility via starch biosynthesis.

Plant physiology·2026
Same journal

The chloroplastic NFU1 maturation factor sustains iron-sulfur cluster assembly in the dark in Chlamydomonas.

Plant physiology·2026
See all related articles

This study examined soybean sieve elements using advanced freeze-substitution and microautoradiography. Findings support an osmotically driven pressure flow mechanism for sucrose translocation in plants.

Area of Science:

  • Plant Biology
  • Cell Biology
  • Biochemistry

Background:

  • Understanding phloem translocation is crucial for plant physiology.
  • Sieve elements are specialized cells responsible for long-distance transport of photoassimilates.
  • Previous studies have debated the structural components and transport mechanisms within sieve elements.

Purpose of the Study:

  • To investigate the ultrastructure of soybean sieve elements.
  • To determine the functional state of sieve elements using microautoradiography.
  • To provide evidence supporting a specific mechanism of phloem translocation.

Main Methods:

  • Quick freezing of soybean petiolar tissue.
  • Freeze-substitution in acetone or propylene oxide.

Related Experiment Videos

  • Epon embedding for electron microscopy and microautoradiography.

    • Main Results:

    • Sieve elements and companion cells showed minimal ice damage.
    • Endoplasmic reticulum (ER) formed stacked clumps along cell walls.
    • Sieve plate pores were largely unobstructed (70%), with some protein fibrils observed.
    • Proteinaceous material near sieve plates consisted of 100 A fibrils.

    Conclusions:

    • The observed structure of sieve elements supports an osmotically generated pressure flow mechanism.
    • The role of protein fibrils in translocation warrants further investigation.
    • Advanced cryo-fixation techniques preserve cellular integrity for accurate ultrastructural analysis.