Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Absorption and long distance transport by isolated stele of maize roots in relation to the dual mechanisms of ion absorption.

Planta·2014

Same author

Cellulase gene expression in ripening avocado fruit: The accumulation of cellulase mRNA and protein as demonstrated by cDNA hybridization and immunodetection.

Plant molecular biology·2013

Same author

Ethylene-regulated gene transcription in carrot roots.

Plant molecular biology·2013

Same author

Gene expression during fruit ripening in avocado.

Planta·2013

Same author

Site-specific binding of a nuclear factor to the carrot extensin gene is influenced by both ethylene and wounding.

Planta·2013

Same author

Identification of a wound-induced inhibitor of a nuclear factor that binds the carrot extensin gene.

Planta·2013

Search research articles

Related Experiment Video

Updated: Jun 24, 2026

High-resolution Respirometry to Assess Mitochondrial Function in Permeabilized and Intact Cells

High-resolution Respirometry to Assess Mitochondrial Function in Permeabilized and Intact Cells

Published on: February 8, 2017

Membrane Lipid Breakdown in Relation to the Wound-induced and Cyanide-resistant Respiration in Tissue Slices: A

A Theologis¹, G G Laties

¹Biology Department and Molecular Biology Institute, University of California, Los Angeles, California 90024.

Plant Physiology

|November 1, 1980

Summary

This summary is machine-generated.

Fresh plant tissues exhibit varied responses to cyanide (CN), with some being sensitive and others resistant. Aging enhances respiration and glucose uptake, suggesting activation of existing capacity rather than new mitochondria.

More Related Videos

In Vivo Imaging of Reactive Oxygen Species in a Murine Wound Model

In Vivo Imaging of Reactive Oxygen Species in a Murine Wound Model

Published on: November 17, 2018

Intra-cardiac Side-Firing Light Catheter for Monitoring Cellular Metabolism using Transmural Absorbance Spectroscopy of Perfused Mammalian Hearts

Intra-cardiac Side-Firing Light Catheter for Monitoring Cellular Metabolism using Transmural Absorbance Spectroscopy of Perfused Mammalian Hearts

Published on: May 12, 2019

Related Experiment Videos

Last Updated: Jun 24, 2026

High-resolution Respirometry to Assess Mitochondrial Function in Permeabilized and Intact Cells

High-resolution Respirometry to Assess Mitochondrial Function in Permeabilized and Intact Cells

Published on: February 8, 2017

In Vivo Imaging of Reactive Oxygen Species in a Murine Wound Model

In Vivo Imaging of Reactive Oxygen Species in a Murine Wound Model

Published on: November 17, 2018

Intra-cardiac Side-Firing Light Catheter for Monitoring Cellular Metabolism using Transmural Absorbance Spectroscopy of Perfused Mammalian Hearts

Intra-cardiac Side-Firing Light Catheter for Monitoring Cellular Metabolism using Transmural Absorbance Spectroscopy of Perfused Mammalian Hearts

Published on: May 12, 2019

Area of Science:

Plant Physiology
Biochemistry
Cellular Respiration

Background:

Plant storage organs and fruits display distinct responses to cyanide (CN), a common inhibitor of cellular respiration.
Freshly cut tissues can be categorized as either CN-sensitive or CN-resistant, influencing their metabolic activity.

Purpose of the Study:

To investigate the differential sensitivity of plant tissue slices to cyanide.
To elucidate the mechanisms underlying wound respiration and aging-induced respiratory changes.
To explore the roles of cytochrome and alternative respiratory pathways in plant tissues.

Main Methods:

Comparative analysis of fresh and aged tissue slices from storage organs and fruits.
Measurement of respiration rates and sensitivity to cyanide (CN).
Assessment of lipid breakdown and glucose utilization.
Investigation of cytochrome and alternative respiratory pathways using uncouplers and inhibitors.

Main Results:

Fresh tissue slices fall into CN-sensitive and CN-resistant categories, with lipid breakdown observed only in sensitive slices.
Slicing induces a significant increase in respiration (wound respiration), further elevated by aging (wound-induced respiration).
Wound respiration is primarily mediated by the cytochrome pathway, while wound-induced respiration involves both cytochrome and alternative pathways in some resistant tissues.
Aging enhances glucose uptake, indicating a transport lesion repair dependent on lipid biosynthesis, not wound respiration.

Conclusions:

Plant tissue responses to cyanide are heterogeneous, linked to lipid metabolism and membrane integrity.
Aging in plant tissues involves activating pre-existing respiratory capacity rather than de novo synthesis of mitochondria.
Membrane integrity is crucial for alternative pathway function, with lipid breakdown leading to its loss.
The repair of glucose transport during aging is independent of wound respiration but relies on lipid biosynthesis.