Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nitrate Utilization by Nitrate Reductase-deficient Barley Mutants.

R L Warner1

  • 1Department of Agronomy and Soils, Washington State University, Pullman, Washington 99164.

Plant Physiology
|April 1, 1981
PubMed
Summary

Nitrate reductase-deficient barley mutants show substantial nitrate reduction despite low enzyme activity. These findings challenge existing understanding of nitrogen assimilation in plants.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Repeatability and reciprocity of the cone optoretinogram.

Biomedical optics express·2023
Same author

Production of Sensitive Monoclonal Antibodies to Aflatoxin B<sub>1</sub>and Aflatoxin M<sub>1</sub> and Their Application to ELISA of Naturally Contaminated Foods.

Journal of food protection·2019
Same author

Inheritance and expression of NAD(P)H nitrate reductase in barley.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Localization of NAD(P)H-bispecific nitrate reductase genes to chromosomes of barley, rye, wheat and Aegilops umbellulata.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

An NADH nitrate reductase gene copy appears to have been deleted in barley.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Vascular tube formation on matrix metalloproteinase-1-damaged collagen.

British journal of cancer·2008

Area of Science:

  • Plant Physiology
  • Molecular Biology
  • Biochemistry

Background:

  • Nitrate reductase is crucial for nitrogen assimilation in plants.
  • Nitrate reductase-deficient mutants are typically chlorate-resistant.
  • Barley mutants were investigated to understand nitrate assimilation pathways.

Purpose of the Study:

  • To investigate nitrate assimilation in barley mutants with deficient nitrate reductase.
  • To assess the growth and nitrogen metabolism of these mutants on different nitrogen sources.
  • To evaluate chlorate resistance in barley nitrate reductase mutants.

Main Methods:

  • Studied two nitrate reductase-deficient barley mutants.
  • Assessed plant growth and nitrogen content using nitrate and ammonium.
  • Measured in vivo and in vitro nitrate reductase activity.
  • Utilized sterile culture of excised embryos to rule out microbial nitrogen sources.
  • Tested chlorate resistance.

Main Results:

  • Barley mutants exhibited low nitrate reductase activity (<10% of control).
  • Mutants showed substantial growth and nitrogen reduction on nitrate as the sole nitrogen source.
  • Mutants displayed only slight resistance to chlorate, unlike typical nitrate reductase-deficient mutants.
  • Sterile embryo culture confirmed mutants' ability to utilize nitrate.

Conclusions:

  • Barley nitrate reductase-deficient mutants are capable of significant nitrate reduction.
  • The study suggests alternative pathways or mechanisms for nitrate assimilation in barley.
  • Findings indicate that nitrate reductase activity levels may not fully correlate with nitrate assimilation capacity in barley.

Related Experiment Videos