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Updated: Aug 8, 2026

Polysome Purification from Soybean Symbiotic Nodules
Published on: July 1, 2022
Spectral evidence for a component involved in hydrogen metabolism of soybean nodule bacteroids
1Laboratory for Nitrogen Fixation Research, Oregon State University, Corvallis, Oregon 97331.
Abstract:
A component with a difference spectrum similar to that of b-type cytochromes which becomes reduced upon the addition of H(2) has been demonstrated in soybean nodule bacteroids. This electron carrier, referred to as component 559-H(2), is present in hydrogenase-positive strains of Rhizobium japonicum but has not been detected in mutants that lack hydrogenase activity or in hydrogenase-negative wild-type strains. A positive correlation between concentrations of component 559-H(2) and hydrogenase activities has been established. These results provide further evidence that component 559-H(2) is involved in H(2) metabolism in R. japonicum.
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