Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nickel is not required for apourease synthesis in soybean seeds.

R G Winkler1, J C Polacco, D L Eskew

  • 1Department of Biochemistry, University of Missouri, Columbia, Missouri 65212.

Plant Physiology
|May 1, 1983
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Two-dimensional active polar semiflexible polymer under shear flow.

The Journal of chemical physics·2026
Same author

Physics of microswimmers--single particle motion and collective behavior: a review.

Reports on progress in physics. Physical Society (Great Britain)·2015
Same author

Arsenate as a potential negative selection agent for deficiency variants in cultured plant cells.

Planta·2013
Same author

Two soybean seed lipoxygenase nulls accumulate reduced levels of lipoxygenase transcripts.

Plant molecular biology·2013
Same author

The inheritance of a urease-null trait in soybeans.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Partitioning of cadmium, copper, lead and zinc amongst above-ground parts of seed and grain crops grown in selected locations in the USA.

Environmental geochemistry and health·2013
Same journal

TaSPL14-D diverged from its ortholog to regulate tiller angle in rice: a caveat for orthology-based functional inference.

Plant physiology·2026
Same journal

From wrinkled seeds to plant oil accumulation networks: The legacy of a Plant Physiology classic.

Plant physiology·2026
Same journal

LcHXK1 mediates glucose signaling to inhibit fruit abscission by phosphorylating LcWRKY42, a feedback regulator in lignin polymerization.

Plant physiology·2026
Same journal

Partial submergence-induced adventitious root emergence in cucumber requires CsRBOHB-mediated ROS production.

Plant physiology·2026
Same journal

JA differentially regulates a SmWLIM1/MYB62-SUS1 module to control male fertility via starch biosynthesis.

Plant physiology·2026
Same journal

The chloroplastic NFU1 maturation factor sustains iron-sulfur cluster assembly in the dark in Chlamydomonas.

Plant physiology·2026
See all related articles

Nickel is not required for soybean urease apoprotein synthesis. However, nickel absorbed by soybean seeds partially activates urease in vivo, demonstrating a crucial role for nickel in urease activity.

Area of Science:

  • Plant biochemistry
  • Enzyme kinetics
  • Nutritional analysis

Background:

  • Soybean urease is a nickel-containing enzyme essential for nitrogen metabolism.
  • Understanding the role of nickel in urease synthesis and activation is critical for crop science.

Purpose of the Study:

  • To investigate the necessity of nickel for urease apoprotein synthesis in soybeans.
  • To determine the mechanism of urease activation by nickel in vivo and in vitro.

Main Methods:

  • Culturing soybean plants (Glycine max cv. Maple Presto) in nickel-free media.
  • Assaying urease activity in nickel-deficient and nickel-sufficient soybeans.
  • Analyzing urease apoprotein levels using biochemical methods.
  • Testing in vitro and in vivo nickel activation of urease.

Related Experiment Videos

Main Results:

  • Nickel-free soybeans exhibited significantly reduced urease activity (<0.005% of nickel-sufficient beans).
  • Nickel-free soybeans contained normal levels of urease apoprotein, indicating nickel is not essential for its synthesis.
  • Urease apoprotein was not activated by nickel in vitro.
  • Partial in vivo activation of urease occurred upon nickel imbibition by seeds, unaffected by cycloheximide.

Conclusions:

  • Nickel is not required for the synthesis of soybean urease apoprotein.
  • Nickel plays a crucial role in the post-translational activation of urease in vivo.
  • The in vivo activation mechanism appears independent of new protein synthesis.