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Updated: Aug 8, 2026
A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
Published on: January 25, 2018
Isolation, purification, and partial characterization of formate dehydrogenase from soybean seed
M P Farinelli1, D W Fry, K E Richardson
1The Ohio State University, Department of Physiological Chemistry, Columbus, Ohio 43210.
Abstract:
Soybean (Glycine soja var Beeson) formate dehydrogenase has been isolated, purified, and partially characterized by affinity chromatography. The enzyme is a dimer having a total molecular weight of 100,000 and a subunit weight of 47,000. It has activity over a broad pH range, is stable for months at 4 degrees C, and has K(m) values of 0.6 millimolar and 5.7 micromolar for formate and NAD, respectively.
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