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Updated: Aug 8, 2026

Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS
Published on: February 24, 2021
Divergent transport mechanisms for pyrimidine nucleosides in petunia pollen
1Department of Agricultural Biochemistry, Waite Agricultural Research Institute, University of Adelaide, Glen Osmond, South Australia 5064 Australia.
Abstract:
Petunia hybrida pollen exhibits divergent transport mechanisms for pyrimidine nucleosides. Uridine and cytidine show all the properties of being actively transported, a nucleoside transport mechanism not hitherto reported in plant cells. Contrasting with this, thymidine transport has the properties of a nonactive, carrier-mediated system. Reasons for these different mechanisms are considered to lie in the high demand for uridine and cytidine, obtained perhaps from stylar tissue, for the biosynthetic reactions of the pollen tube, while thymidine demand is lower due to the absence of DNA replication in germinating Petunia pollen.
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