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Related Experiment Videos

Changes in Gene Expression during Tomato Fruit Ripening.

M S Biggs1, R W Harriman, A K Handa

  • 1Department of Horticulture, Purdue University, West Lafayette, Indiana 47907.

Plant Physiology
|June 1, 1986
PubMed
Summary
This summary is machine-generated.

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This study investigates protein and mRNA changes during tomato fruit ripening, comparing normal Rutgers tomatoes with ripening-impaired mutants. Key polypeptide and mRNA alterations were identified, revealing molecular insights into tomato fruit development.

Area of Science:

  • Plant Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Tomato fruit ripening involves complex genetic and molecular changes.
  • Understanding these changes is crucial for improving fruit quality and shelf life.
  • Mutants like rin, nor, and Nr offer valuable insights into ripening regulation.

Purpose of the Study:

  • To analyze protein and mRNA expression profiles during normal tomato fruit ripening.
  • To compare these profiles with those of ripening-impaired tomato mutants (rin, nor, Nr).
  • To identify specific polypeptides and mRNAs associated with ripening progression.

Main Methods:

  • Extraction and separation of total proteins using SDS-PAGE.
  • Isolation and fractionation of total RNAs into poly(A)(+) and poly(A)(-) fractions.

Related Experiment Videos

  • In vitro translation of poly(A)(+) RNAs using rabbit reticulocyte lysates.
  • Immunoprecipitation of specific proteins, such as polygalacturonase precursor.

    • Main Results:

    • Significant changes in polypeptide levels (increases, decreases, fluctuations) were observed during normal ripening.
    • Specific polypeptides were found at low or undetectable levels in ripening-impaired mutants.
    • mRNA populations also exhibited dynamic changes, with distinct increases, decreases, and fluctuations in translatable mRNAs.
    • A polygalacturonase precursor was detected in various ripening stages and in the Nr mutant.

    Conclusions:

    • Tomato fruit ripening is characterized by extensive, coordinated changes in both protein and mRNA populations.
    • Ripening-impaired mutants display altered protein and mRNA profiles compared to normal fruit.
    • These findings contribute to a deeper understanding of the molecular mechanisms regulating tomato fruit development and ripening.