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Published on: October 5, 2016
The MADS-box transcription factor VvSVP1 negatively regulates grapevine bud dormancy release
Pinqi Sun1, Anni Chen1, Hantang Huang1
1Department of Fruit Tree Sciences, College of Horticulture, China Agricultural University, Beijing 100193, China.
Grapevine bud dormancy is negatively regulated by VvSVP1, a MADS-box transcription factor. Silencing VvSVP1 promotes bud break by affecting abscisic acid metabolism and meristem activation, offering insights into fruit tree dormancy.
Area of Science:
- Plant Biology
- Molecular Genetics
- Agricultural Science
Background:
- Grapevine (Vitis vinifera L.) bud dormancy release is crucial for fruit production but sensitive to climate.
- Understanding the molecular basis of dormancy is key to managing bud break.
Purpose of the Study:
- To investigate the role of the MADS-box transcription factor VvSVP1 in regulating grapevine bud dormancy release.
- To elucidate the molecular mechanisms by which VvSVP1 controls bud break.
Main Methods:
- Transient gene silencing and overexpression of VvSVP1 in grapevine.
- Analysis of VvSVP1 interaction with target gene promoters (VvCYP707A4, VvFT).
- Transcriptome analysis and identification of upstream regulators (VvABF2).
Main Results:
- VvSVP1 negatively regulates bud dormancy; silencing promotes bud break, while overexpression delays it.
- VvSVP1 directly inhibits the expression of VvCYP707A4 (ABA catabolism) and VvFT (florigen).
- VvABF2 acts as a positive regulator upstream of VvSVP1, influencing bud break and VvSVP1 expression.
Conclusions:
- A regulatory model for grapevine bud dormancy release centered on VvSVP1 is proposed.
- This study provides a foundation for developing eco-friendly dormancy-releasing agents.
- Findings contribute to understanding bud dormancy mechanisms in other fruit trees.
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