Related Experiment Video
Updated: Aug 8, 2026
Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers
Published on: March 4, 2021
Identification of endogenous gibberellins from sorghum
S B Rood1, K M Larsen, L N Mander
1Department of Biological Sciences, University of Lethbridge, Lethbridge, Alberta T1K 3M4, Canada.
Abstract:
Gibberellins (GA) A(1), A(19), and A(20) were identified in shoot cylinders containing the apical meristems from sorghum (Sorghum bicolor L.). Extracts were purified by sequential SiO(2) partition chromatography and reversed-phase C(18) high performance liquid chromatography and biologically active (dwarf rice cv Tan-ginbozu microdrop assay) fractions were subjected to gas chromatography-selected ion monitoring. Based on the use of [(3)H]GA and [(2)H](d(2))GA internal standards, amounts of GA(1), GA(19), and GA(20) were estimated to be 0.7, 8.8, and 1.5 namograms per gram dry weight of tissue, respectively.
