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Related Experiment Videos

Phytochrome Photoconversion in Vivo: Comparison between Measured and Predicted Rates.

A L Mancinelli1

  • 1Department of Biological Sciences, Columbia University in the City of New York, New York, New York 10027.

Plant Physiology
|March 1, 1988
PubMed
Summary

Phytochrome photoconversion rates in plants differ from predictions, influenced by light reflection and sample orientation. White surfaces enhance these rates, while black surfaces reduce them, impacting plant photomorphogenesis.

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Area of Science:

  • Plant Physiology
  • Photobiology
  • Spectroscopy

Background:

  • Phytochrome is a key photoreceptor regulating plant growth and development.
  • Accurate prediction of phytochrome photoconversion rates is crucial for understanding photomorphogenesis.
  • In vivo measurements often deviate from theoretical models based on purified phytochrome.

Purpose of the Study:

  • To investigate discrepancies between measured and predicted phytochrome photoconversion rates in vivo.
  • To determine the influence of light source geometry and reflective surfaces on phytochrome photoconversion.
  • To elucidate the role of light reflection in modulating phytochrome activity in plants.

Main Methods:

  • Measuring phytochrome photoconversion rates in etiolated cabbage seedlings and cucumber cotyledons under various light conditions (blue, red, far-red).

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  • Analyzing the impact of sample orientation (flat vs. vertical) and substrate reflectivity (white vs. black filter paper) on photoconversion rates.
  • Comparing in vivo measured rates with predictions based on spectral photon flux and purified phytochrome optical parameters.

    • Main Results:

    • Measured phytochrome photoconversion rates in vivo significantly differed from predicted rates.
    • Sample geometry and light reflection substantially affected photoconversion rates.
    • Phytochrome photoconversion was significantly faster in cabbage seedlings on white, wet filter paper compared to vertical seedlings.
    • Replacing white filter paper with black significantly decreased photoconversion rates in cucumber cotyledons.

    Conclusions:

    • Light reflection and geometrical factors are critical, unaddressed variables in phytochrome photoconversion models.
    • In vivo phytochrome activity is highly sensitive to the immediate reflective environment.
    • Future studies must incorporate these environmental factors for accurate photomorphogenesis research.