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Related Experiment Videos

Salinity affects intracellular calcium in corn root protoplasts.

J Lynch1, A Läuchli

  • 1Department of Land, Air and Water Resources, University of California, Davis, California 95616.

Plant Physiology
|June 1, 1988
PubMed
Summary

Salinity displaces calcium from corn root cell membranes, impacting cellular processes. This study reveals how sodium chloride affects calcium localization and membrane fluidity in plant roots.

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Area of Science:

  • Plant Physiology
  • Cell Biology
  • Biochemistry

Background:

  • Salinity stress affects plant growth and development.
  • Calcium ions (Ca) play crucial roles in plant cell signaling and membrane stability.
  • Previous studies indicated salinity displaces Ca from root cell membranes using chlorotetracycline (CTC).

Purpose of the Study:

  • To investigate whether salinity displaces Ca from the cell surface or internal membranes of corn (Zea mays L.) root protoplasts.
  • To elucidate the mechanisms by which salinity affects Ca distribution and membrane properties.

Main Methods:

  • Utilized the fluorescent Ca probe chlorotetracycline (CTC) to monitor Ca localization.
  • Employed supplemental Ca preloading, EGTA quenching, and Ca channel reagents to assess Ca displacement.

Related Experiment Videos

  • Measured ion influx/efflux and fluorescence anisotropy to evaluate membrane fluidity and ion transport.

    • Main Results:

    • Supplemental Ca counteracted NaCl effects on CTC fluorescence, suggesting intracellular Ca pools are involved.
    • NaCl's effect on CTC fluorescence was modulated by Ca channel reagents, Li pretreatment, and inositol.
    • Salinity increased Na influx, decreased Ca influx, and increased Ca efflux.
    • NaCl altered membrane fluidity, decreasing it at the plasmalemma's external face but increasing it in general.

    Conclusions:

    • Salinity displaces intracellular Ca, likely through phosphoinositide system activation and depletion of intracellular Ca pools.
    • These changes in Ca distribution and membrane fluidity contribute to salinity stress responses in corn roots.