Identification of endogenous gibberellins in navel orange shoots

Eight gibberellins (GAs) were identified from vegetative shoots of navel orange trees (Citrus sinensis L. Osbeck cv Washington) after sequential purification by reverse-phase C(18) high performance liquid chromatography, Nucleosil 5N(CH(3))(2) high performance liquid chromatography, and capillary gas chromatography-mass spectrometry. GA(1), GA(17), GA(19), GA(20), GA(29), and iso-GA(3) were identified based on the full scan mass spectra and Kovats retention indices. GA(8) was tentatively identified based on the comparison of the full scan mass spectra with the published spectra. GA(44) was tentatively identified from the characteristic masses at the correct Kovats retention index.