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Electron microscopic observation of recombinant Escherichia coli cells overproducing human tumor necrosis
H Moriya1, J Kaneko, S Nakamura
1Material Analysis Research Center, Teijin Limited, Tokyo, Japan.
Abstract:
Escherichia coli C600 r-m- carrying plasmid pTNF483 (E. coli [pTNF483]) produces a tumor necrosis factor-alpha (TNF-alpha) mutant protein in an insoluble form. A swollen region was observed in the SEM images to encircle the outside of most of the E. coli [pTNF483] cells just like a bandage. On the other hand, inclusion bodies of the TNF-alpha mutant as large as the short axis of the cell were observed in TEM images. This position was regarded as coinciding with the swollen region of SEM images. The inclusion bodies revealed in the swollen region of the cell envelopes were clearly observed in SEM images of isolated insoluble structures obtained by centrifugal sedimentation of a sonicated cell suspension.