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Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana
Published on: January 5, 2018
Protein synthesis in isolated castor bean mitochondria is stimulated by cyanide
M A Kaderbhai1, R B Beechey, N Kaderbhai
1Department of Biochemistry, School of Agricultural Sciences, The University College of Wales, Aberystwyth, Penglais, Aberystwyth, Dyfed SY23, 3DD Wales, United Kingdom.
Abstract:
Cyanide added to isolated castor bean (Ricinus communis L.) mitochondria supplemented with ATP and succinate (or NADH) significantly enhanced the rate and extent of organellar protein synthesis. Cyanide stimulated mitochondrial protein synthesis in a dose-dependent manner with an optimum stimulation of over twofold at 1 millimolar cyanide. At this concentration of cyanide, the mitochondrial respiratory activity, in the presence of succinate (or NADH) and ADP was inhibited by 90%. The stimulatory effect of cyanide on mitochondrial translation was reflected in the increased synthesis of all the proteins synthesized within the organelle. Preliminary evidence indicates a role for the alternative, salicylhydroxamic acid-sensitive, oxidase in the cyanide stimulation of protein synthesis.
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