Related Experiment Video
Updated: Aug 8, 2026
14:16
A New Application of the Electrical Penetration Graph (EPG) for Acquiring and Measuring Electrical Signals in Phloem Sieve Elements
Published on: July 2, 2015
Ca-selective microelectrodes and their application to plant cells and tissues
1Institut für Botanik I der Justus-Liebig-Universität, Senckenbergstrasse 17-21, D-6300 Giessen, Federal Republic of Germany.
Plant Physiology
|December 1, 1989
Summary
Fabricating calcium-selective (Ca2+) microelectrodes for plant physiology presents challenges. Recent advances in microelectrode design and fabrication improve their utility for intracellular calcium measurements in plant cells.
Area of Science:
- Plant Physiology
- Biophysical Chemistry
- Cell Biology
Background:
- Calcium-selective (Ca2+) microelectrodes are crucial for studying intracellular calcium dynamics.
- Existing microelectrodes face challenges including non-Nernstian slopes, slow response times, and tip damage in plant cells.
Purpose of the Study:
- To review recent advancements in the fabrication of Ca2+-selective single and double-barreled microelectrodes.
- To discuss the intracellular applications of these improved microelectrodes in various plant cell types.
Main Methods:
- Fabrication techniques including beveling electrodes, incorporating sensor components with polyvinylchloride, insulating hydrated glass, and stabilizing tips.
- Application of microelectrodes for intracellular Ca2+ measurements in plant cells.
Main Results:
- Developed microelectrodes exhibit improved performance, overcoming issues like non-Nernstian slopes and slow responses.
- Successful intracellular Ca2+ measurements were achieved in diverse plant cell materials.
Conclusions:
- Despite experimental demands, Ca2+-selective microelectrodes are valuable tools for plant physiology research.
- Ongoing progress in fabrication enhances the reliability and applicability of these electrodes for in vivo studies.
