Genotoxicity evaluation of five different dentin bonding agents by chromosomal aberration analysis

Dentin bonding agents became unavoidable in today's aesthetic restorative dentistry. Nevertheless, more and more evidences on their possible cytotoxicity and/or genotoxicity emerge. Still, only limited number of studies has been published on that issue. In our work we evaluated possible genotoxicity of five different adhesives: Adper Single Bond, Adper Single Bond 2 with nanofiller, Excite, OptiBond Solo Plus and Prompt L-pop. Genotoxicity assessment was carried out on human lymphocytes in vitro, using chromosomal aberration analysis. Polymerized adhesives were tested at three different dilutions of the 0.5 g mL(-1) eluate stock (2.5 x 1:10(6), 1:10(6) and 1:10(5)) after 1 h, 24 h and 5 days of elution. Slight but significant increase in the number of chromatid breaks was observed after 24-h elution period, for adhesives Adper Single Bond 2, Excite, and OptiBond Solo Plus at dilutions of 1:10(6) and 1:10(5), and for other two only at dilution of 1:10(5). First three adhesives also appeared to be slightly genotoxic after 1 h of elution but only at 1:10(5). As a bonding agent remains in close contact with living dental tissue over a long period of time, information on their possible genotoxicity and carcinogenicity should be more clearly clarified in the near future.