Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A self-assembling process in articular cartilage tissue engineering.

Jerry C Hu1, Kyriacos A Athanasiou

  • 1Department of Bioengineering, Rice University, Houston, Texas 77005, USA.

Tissue Engineering
|May 6, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Nondestructive Raman Spectral Method for Temporal Tracking of Articular Cartilage Maturation.

Tissue engineering. Part A·2026
Same author

Isolation, purification and characterization of lipocartilage in mice.

Nature protocols·2026
Same author

Minipig costal and knee cartilage structure-function relationships and their use as cell sources for tissue-engineered analogous cellular products for cartilage repair.

Acta biomaterialia·2025
Same author

Preclinical perspectives on disorders of the temporomandibular joint: Tracing the past, navigating the present, and shaping the future.

The journal of pain·2025
Same author

Characterization of the temporomandibular joint of the gray wolf (Canis lupus) in health and disease.

Journal of comparative pathology·2025
Same author

Formation of functionally robust human neocartilage from multiple donors using highly expanded costochondral cells.

Biofabrication·2025
Same journal

Change in u.s. Patent infringement law.

Tissue engineering·2009
Same journal

Functional reconstruction of the jaw bones using poly(l-lactide) mesh and autogenic particulate cancellous bone and marrow.

Tissue engineering·2009
Same journal

Effect of the structure of bone morphogenetic protein carriers on ectopic bone regeneration.

Tissue engineering·2009
Same journal

Cross-linking of gelatin with carbodiimides.

Tissue engineering·2009
Same journal

Early treatment of diabetes with porcine islets in a bioartificial pancreas.

Tissue engineering·2009
Same journal

Permeability of filters used for immunoisolation.

Tissue engineering·2009
See all related articles

This study introduces a novel scaffold-free self-assembly method for engineering hyaline articular cartilage. The process yields tissue-engineered cartilage with properties approaching native tissue, offering a promising alternative to current therapies.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine
  • Orthopedic Surgery

Background:

  • Current treatments for articular cartilage defects often lead to suboptimal fibrocartilage formation.
  • Existing tissue-engineering strategies using scaffolds face challenges like cell phenotypic alteration and material toxicity.
  • There is a critical need for advanced methods to regenerate hyaline articular cartilage.

Purpose of the Study:

  • To investigate a scaffold-free self-assembling process for creating hyaline articular cartilage constructs.
  • To evaluate the histological, biochemical, and biomechanical properties of self-assembled cartilage.
  • To compare the efficacy of this method against traditional scaffold-based approaches.

Main Methods:

Related Experiment Videos

  • Utilized a self-assembling process in vitro, without the use of traditional scaffolds.
  • Constructs were cultured over agarose for 12 weeks.
  • Assessed GAG and collagen content, collagen type I and II production (IHC, gel electrophoresis), and biomechanical properties (stiffness).

Main Results:

  • Self-assembled constructs exhibited hyaline-like appearance and properties comparable to native articular cartilage.
  • Achieved significantly higher glycosaminoglycan (GAG) and collagen content than calf articular cartilage.
  • Demonstrated production of collagen type II and absence of collagen type I, with stiffness exceeding one third of native tissue.

Conclusions:

  • The scaffold-free self-assembly method is a viable approach for engineering hyaline articular cartilage.
  • This technique overcomes limitations associated with traditional scaffolds, yielding superior tissue regeneration.
  • The resulting constructs possess promising biomechanical and biochemical characteristics for cartilage repair.