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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Growth of Cartilage and Bone Tissue01:27

Growth of Cartilage and Bone Tissue

Chondrocytes form a temporary cartilaginous model by dividing and secreting a thick gel-like extracellular matrix. Once the chondrocytes undergo programmed cell death, osteoblasts enter the site of the cartilaginous model. The process of replacing the temporary cartilaginous model with bone in an ordered manner is called endochondral ossification. In endochondral ossification, not all of the cartilage is replaced by bone tissue. Some cartilage that performs a protective and supportive function...

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Related Experiment Video

Updated: Jun 24, 2026

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

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Published on: May 29, 2012

A Nondestructive Raman Spectral Method for Temporal Tracking of Articular Cartilage Maturation.

Nathan J Castro1, Greta Babakhanova2, Ryan Donahue1

  • 1Department of Biomedical Engineering, University of California-Irvine, Irvine, California, USA.

Tissue Engineering. Part A
|June 23, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed a Raman spectroscopy method to non-destructively assess tissue-engineered cartilage development. This technique enables rapid quality control for biomanufacturing, ensuring product consistency and efficacy.

Keywords:
Maturity IndexRaman spectroscopycartilagenondestructive testingspectropathologytemporal trackingtissue-engineered cartilage

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Area of Science:

  • Biotechnology
  • Biomaterials Science
  • Spectroscopy

Background:

  • Articular cartilage (AC) has limited self-repair capabilities, driving research into tissue engineering for clinical applications.
  • Current manufacturing of engineered tissues requires rapid, non-destructive methods for in-process and release quality assessment.

Purpose of the Study:

  • To introduce a Raman spectroscopy-based methodology for non-destructive qualitative and quantitative characterization of tissue development in AC.
  • To establish spectroscopic biomarkers for monitoring tissue maturation and enabling quality control in biomanufacturing.

Main Methods:

  • Collated Raman shifts for key AC biochemical components: DNA, glycosaminoglycans, total collagen, and pyridinoline.
  • Tracked temporal maturation of nascent and mature AC to create a reference dataset for spectroscopic biomarkers.
  • Validated spectroscopic biomarkers against traditional photometric assays and mass spectrometry.

Main Results:

  • Developed a 'Maturity Index' for quantifying tissue development and maturation.
  • Achieved strong correlations (R² > 0.96) between non-destructive Raman measurements and destructive biochemical assays (R² > 0.97).
  • Demonstrated the potential for in-line, non-destructive quality assessment during the manufacturing of engineered tissues.

Conclusions:

  • Raman spectroscopy is a powerful tool for non-destructive quality control and assurance in biomanufacturing.
  • The developed methodology provides a rapid and reliable approach for monitoring tissue-engineered medical product development.
  • This approach can serve as a template for studying the development of other native and engineered tissues.