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Published on: November 19, 2016
Allergenicity of 10 different apple varieties
Jerónimo Carnés1, Angel Ferrer, Enrique Fernández-Caldas
1Laboratorios LETI, SL Research Laboratories, Tres Cantos, Madrid, Spain.
Summary
This study reveals significant differences in apple allergen profiles, particularly for Mal d 3, impacting oral allergy syndrome. Starking apples showed the highest allergenicity, while Reineta Parda and Royal Gala were least allergenic.
Area of Science:
- Food allergy research
- Immunology
- Plant biochemistry
Background:
- Over 100 apple varieties exist globally, but their allergenic profiles remain largely uncharacterized.
- Understanding apple variety allergenicity is crucial for managing food allergies.
Purpose of the Study:
- To comprehensively analyze the antigenic and allergenic profiles of 10 distinct commercial apple varieties.
- To investigate variations in key allergens like Mal d 3 across different apple types.
Main Methods:
- Apple peels from 10 varieties were extracted and analyzed using SDS-PAGE and immunoblotting.
- Specific IgE levels and skin prick tests were performed on patients with oral allergy syndrome.
- Protein content and allergenicity were quantified using established biochemical and immunological methods.
Main Results:
- Significant differences in antigenic profiles were observed among the 10 apple varieties.
- A 9-kDa band, likely Mal d 3, was present in all extracts, with notable variations in content.
- Starking apples exhibited the highest protein content and allergenicity, while Reineta Parda and Royal Gala showed the lowest.
Conclusions:
- Apple varieties possess distinct antigenic and allergenic profiles, with significant variations in Mal d 3 content.
- These findings highlight the importance of considering apple variety when assessing allergy risks.
