[Indirect ELISA for the pyrethroids-permethrin residue detection]

Area of Science:

• Environmental Chemistry
• Immunochemistry
• Analytical Chemistry

Context:

• Permethrin is a widely used insecticide with potential environmental persistence.
• Accurate detection methods are crucial for environmental monitoring and risk assessment.
• Developing sensitive and reliable assays for pesticide residue analysis is an ongoing challenge.

Purpose:

• To establish an indirect competitive ELISA (icELISA) for the sensitive detection of permethrin residue in environmental samples.
• To optimize the icELISA assay parameters, including coating antigen titration and dilution.
• To validate the performance of the developed icELISA, assessing its detection limit, linearity, and recovery rates.

Summary:

• Ovalbumin (OVA) was conjugated with permethrin using the active ester method to create a semi-antigen.
• An indirect competitive ELISA (icELISA) was successfully established and optimized, with the best coating antigen titration at 0.45 μg/mL and dilution of 1:2500.
• The icELISA exhibited a low detection limit of 0.1 μg/mL, a favorable linear relationship between 10-800 μg/mL, and satisfactory permethrin recoveries exceeding 97%.

Impact:

• Provides a sensitive and reliable method for detecting permethrin residues in environmental matrices.
• Facilitates environmental monitoring programs aimed at assessing permethrin contamination.
• Contributes to the development of immunochemical tools for pesticide analysis, supporting environmental protection efforts.