Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

When a sperm meets an egg: block to polyspermy.

Alina Tsaadon1, Efrat Eliyahu, Nataly Shtraizent

  • 1Department of Cell & Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, Israel.

Molecular and Cellular Endocrinology
|May 12, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metabolic gene therapy in a canine with pulmonary hypertension secondary to degenerative mitral valve disease.

Frontiers in veterinary science·2024
Same author

Author Correction: The direct effect of SARS-CoV-2 virus vaccination on human ovarian granulosa cells explains menstrual irregularities.

NPJ vaccines·2024
Same author

The direct effect of SARS-CoV-2 virus vaccination on human ovarian granulosa cells explains menstrual irregularities.

NPJ vaccines·2024
Same author

Pigment epithelium-derived factor expression and role in follicular development.

Reproductive biomedicine online·2024
Same author

Unilateral Lung Removal in Combination with Monocrotaline or SU5416 in Rodents: A Reliable Model to Mimic the Pathology of the Human Pulmonary Hypertension.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Gene delivery in a rodent lung transplant model.

The Journal of thoracic and cardiovascular surgery·2024

Cortical granule exocytosis (CGE) prevents polyspermy during egg activation. Myristoylated alanine-rich C kinase substrate (MARCKS) protein dissociation from actin facilitates CGE, potentially improving assisted reproduction outcomes.

Area of Science:

  • Reproductive Biology
  • Cell Biology
  • Developmental Biology

Background:

  • Egg activation initiates embryonic development, involving calcium increase and cortical granule exocytosis (CGE).
  • CGE modifies the zona pellucida, preventing polyspermy and ensuring successful fertilization.
  • The egg's CGE is a unique model for studying membrane fusion cascades, occurring only once per egg.

Purpose of the Study:

  • To highlight proteins involved in cortical granule exocytosis (CGE) and the block to polyspermy.
  • To investigate the role of filamentous actin (F-actin) and myristoylated alanine-rich C kinase substrate (MARCKS) in CGE.

Main Methods:

  • Examined CGE triggered by calcium-dependent pathways or protein kinase C (PKC) activation.
  • Investigated the dynamic nature of the egg's cortical F-actin network.

Related Experiment Videos

  • Assessed MARCKS expression, colocalization with actin, and dissociation upon ionomycin-induced egg activation.

    • Main Results:

    • MARCKS is expressed and colocalizes with F-actin in non-activated MII eggs.
    • Ionomycin-induced egg activation leads to MARCKS dissociation from actin.
    • This dissociation potentially causes F-actin network breakdown, facilitating CGE.

    Conclusions:

    • The dynamic F-actin network, regulated by proteins like MARCKS, plays a crucial role in CGE.
    • Understanding CGE mechanisms and participating proteins can advance assisted reproductive technologies.
    • MARCKS dissociation from actin is a key event enabling CGE and preventing polyspermy.