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Updated: Aug 10, 2026

Hyperinsulinemic-euglycemic Clamps in Conscious, Unrestrained Mice
Published on: November 16, 2011
[Multiple insulin injections with the help of a subcutaneous catheter or metered syringe (Pen)]
J Tatoń1, A Czech, E Jagielińska-Kalinowska
1Katedry i Kliniki Chorób Wewnetrznych i Diabetologii AM, Szpital Bródnowski w Warszawie.
Abstract:
Multiple, daily injections of insulin in intensified insulin therapy of diabetes mellitus has many important advantages. From the technical point of view however it requires new instruments, which could further limit the trauma of injection and facilitate the necessary manipulations. For these reasons a combination of typical insulin syringe (Novo-Noraisk Pen) and a special subcutaneous insulin catheter (Vigo-Spectramed Insuflon) was examined in a systematic way. Insuflon was inserted for 7 days. Insulin injection was made with a syringe through the head of the Insuflon. The needle of a syringe easily penetrated the membrane inside the Insuflon 4 times daily for 7 days without any damage. The acceptance of this method was high. It increased the compliance in general. The parameters of diabetes mellitus control changed positively. No side-effects of such procedure were noted.
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