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Updated: Aug 8, 2026
Preparation of Functional Silica Using a Bioinspired Method
Published on: August 1, 2018
Covalent immobilization of triacylglycerol lipase onto functionalized novel mesoporous silica supports
Yong-Xiao Bai1, Yan-Feng Li, Yong Yang
1College of Chemistry and Chemical Engineering, State Key Laboratory of Applied Organic Chemistry, Institute of Biochemical Engineering and Environmental Technology, Lanzhou University, Lanzhou 730000, PR China.
Abstract:
A novel mesoporous silica material was synthesized via a silicate salt route in the presence of polyvinyl alcohol as the structure-directing agent under acidic conditions. The material was functionalized and employed as the supports (LPS-1 and LPS-2) for immobilizing triacylglycerol lipase from porcine pancreas (PPL). Not only they had a good thermal stability and reusability but also the activity recovery of LPS-1 and LPS-2 reached to 69% and 76%, respectively. The optimal pH and temperature region of the LPS supports immobilized PPL for hydrolysis of olive oil were at 8.0 and 55-60 degrees C. Kinetic parameters such as maximum velocity (V(max)) and the Michaelis constant (K(m)) were determined for the free and the immobilized lipase and LPS-2 immobilized PPL had the highest catalytic efficiency in the three. Meanwhile, the LPS supports exhibited many advantages than small porous materials for immobilizing PPL.
