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Analysis of paraphenylenediamine
M A Bellimam1, A Stambouli, N El Karni
1Laboratoire de Recherches et d'Analyses et Scientifiques de la Gendarmerie Royale, BP. 6597, Rabat-lnstituts, CP 10100 Rabat-Maroc.
Abstract:
The toxic Paraphenylenediamine is characterized by infrared spectrophotometry and nuclear magnetic resonance spectroscopy, whereas, high performance liquid chromatography with a refractive index detector was used to determine its purity in the suspect samples using external standardisation. An analytical method for determination of lower traces of paraphenylenediamine in post-mortem biological fluids was developed. This procedure involves deproteneization or hydrolysis followed by liquid-liquid extraction and derivatization with trifluoroacetic anhydride. 1 microL of the extract was then analysed by gas chromatography/iontrap mass spectrometry. Benzidine used as the internal standard for quantification and the extraction recovery test was evaluated to 85%. This method was validated in cases with paraphenylenediamine poisoning.
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