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Published on: April 13, 2016

Matrix metalloproteinase-9 activity in plasma correlates with plasma and whole blood lead concentrations

Fernando Barbosa1, Raquel F Gerlach, Jose E Tanus-Santos

  • 1Department of Clinical, Toxicological and Food Science Analysis, Faculty of Pharmaceutical Sciences of Ribeirao Preto, University of Sao Paulo, Av. do Cafe s/n, S.P. 14040-903, Ribeirao Preto, SP, Brazil.

Basic & Clinical Pharmacology & Toxicology
|May 17, 2006
PubMed

Insights

Lead exposure is linked to increased matrix metalloproteinase-9 (MMP-9) activity, potentially increasing susceptibility to cardiovascular and neoplastic diseases. Further research is needed to confirm a causal relationship.

Area of Science:

  • Biochemistry
  • Toxicology
  • Cardiovascular Science

Background:

  • Matrix metalloproteinases (MMPs), particularly MMP-2 and MMP-9, are crucial in extracellular matrix degradation.
  • Elevated MMP-9 levels are associated with cardiovascular and neoplastic diseases, serving as potential diagnostic and prognostic markers.
  • Lead exposure is a known health concern with various toxicological implications.

Purpose of the Study:

  • To investigate the association between plasma activities of MMP-2 and MMP-9 and lead concentrations in blood and plasma.
  • To explore potential mechanisms linking lead exposure to diseases associated with MMPs.

Main Methods:

  • Plasma MMP-2 and MMP-9 activities were measured using gelatin zymography.
  • Lead concentrations in whole blood (blood Pb) and plasma (plasma Pb) were determined using graphite furnace atomic absorption spectrometry (GF-AAS) and inductively coupled plasma mass spectrometry (ICP-MS), respectively.
  • Correlation analyses were performed on data from 40 lead-exposed individuals.

Main Results:

  • A significant positive correlation was observed between plasma pro-MMP-9 activity and both blood Pb (r=0.454; P=0.003) and plasma Pb (r=0.312; P=0.049).
  • No significant correlations were found between lead concentrations (blood or plasma) and plasma MMP-2 activity.
  • These findings suggest a link between lead exposure and elevated MMP-9 activity.

Conclusions:

  • The association between pro-MMP-9 activity and lead levels suggests a potential mechanism by which lead exposure may heighten susceptibility to cardiovascular and neoplastic diseases.
  • While a correlation is established, a causal relationship between lead exposure and increased MMP-9 activity requires further investigation.
  • This study highlights a potential biomarker role for MMP-9 in lead-related health risks.

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