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[Quantitative study of osteoclastic related factors in the process of bone reconstruction]
Xin Chang1, Zhi-Ming Hou, Yasuaki Sibata
1Dept. of Oral Science, The Second Affiliated Hospital of Dalian Medical University, Dalian 116023, China.
Objective:
To detect the expression of osteoclast related factors, tumor necrosis factor-alpha (TNF-alpha), receptor activator of NF-kappaB ligand (RANKL) and tartrate-resistant acid phosphatase (TRAP), in the process of bone remodeling.
Methods:
8-week-old male C57BL/6J mice were employed in this study to detect the expression of osteoclast related factors by real time PCR.
Results:
TNF-alpha, RANKL and TRAP were up regulated in the process of bone remodeling, they reached the peak on day 2, 5 and 10 individually after injury.
Conclusion:
Osteoclast related factors also participate in bone remodeling, which depends on the delicate balance between bone formation and bone resorption.
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