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Related Experiment Videos

Human polymorphonuclear leukocytes produce and express functional tissue factor upon stimulation.

N Maugeri1, M Brambilla, M Camera

  • 1Research Laboratories, Center for High Technology Research and Education in Biomedical Sciences, Catholic University, Campobasso, Italy. normamaugeri2003@yahoo.it

Journal of Thrombosis and Haemostasis : JTH
|May 19, 2006
PubMed
Summary

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Polymorphonuclear leukocytes (PMN) express tissue factor (TF) upon stimulation. This suggests inflammatory cells and platelets are key in thrombus formation.

Area of Science:

  • Hematology
  • Immunology
  • Molecular Biology

Background:

  • Blood-borne tissue factor (TF) is critical in initiating blood clots (thrombogenesis).
  • Investigating the cellular sources of TF is essential for understanding thrombotic events.

Purpose of the Study:

  • To determine if polymorphonuclear leukocytes (PMN) serve as a source of tissue factor (TF).

Main Methods:

  • Human PMN were isolated and stimulated with P-selectin or fMLP.
  • TF procoagulant activity and TF antigen (TF:Ag) were measured using TF MoAb and flow cytometry.
  • TF mRNA levels were assessed via real-time PCR (RT-PCR).

Main Results:

  • Stimulated PMN exhibited increased TF procoagulant activity and TF:Ag expression (4-6 fold).

Related Experiment Videos

  • Approximately 40% of PMN contained TF:Ag, with stimulation affecting its cellular localization.
  • TF mRNA levels significantly increased after 1-hour stimulation.

    • Conclusions:

    • TF is not constitutively expressed in peripheral PMN but is upregulated upon stimulation.
    • Activated PMN rapidly express functional TF on their surface, contributing to thrombus formation.
    • PMN-derived TF highlights the role of inflammatory cells and platelet interactions in fibrin deposition.