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Preparation of Living Isolated Vertebrate Photoreceptor Cells for Fluorescence Imaging
Published on: June 22, 2011
The optics of vertebrate photoreceptors: anisotropy and form birefringence
1School of Physics and Astronomy, University of Manchester, Manchester M13 9PL, UK. nicholas.roberts@manchester.ac.uk
Abstract:
The optics of vertebrate photoreceptors have been investigated with specific reference to the effect of form birefringence. The complex dielectric tensor of the lamellar-like outer segment structure has been derived, allowing the transverse spectral absorbance to be calculated for different incident polarizations. These results were used to calculate the changes in the cellular dichroic ratio as a function of both the volume occupied by the bilayers and the real and complex parts of the intrinsic birefringence of the bilayers. Physiologically realistic values of these parameters show the cellular dichroic ratio to be greater than the bilayer dichroic ratio by a factor of approximately 1.3. Furthermore, the calculations of spectral absorbance indicate that form birefringence may affect measurements of optical density in transversely orientated outer segments.
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Isomers are different chemical species that have the same chemical formula.
Transition metal complexes often exist as geometric isomers, in which the same atoms are connected through the same types of bonds but with differences in their orientation in space. Coordination complexes with two different ligands in the cis and trans positions from a ligand of interest form isomers. For example, the octahedral [Co(NH3)4Cl2]+ ion has two isomers (Figure 1) In the cis...
