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Related Experiment Videos

Influence of different storage methods on laser fluorescence values: a two-year study.

P Francescut1, B Zimmerli, A Lussi

  • 1Department of Operative, Paediatric and Preventive Dentistry, School of Dental Medicine, University of Bern, Bern, Switzerland. paola_francescut_hase@hotmail.com

Caries Research
|May 19, 2006
PubMed
Summary

Dental storage solutions significantly alter infrared laser fluorescence. Frozen teeth maintain stable fluorescence for in vitro studies, allowing reliable extrapolation to clinical applications.

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Area of Science:

  • Dentistry
  • Biomaterials Science
  • Optical Diagnostics

Background:

  • Infrared laser fluorescence is a valuable diagnostic tool in dentistry.
  • Standardized storage methods are crucial for reliable in vitro dental research.
  • Previous research has not fully elucidated the impact of various storage solutions on tooth fluorescence.

Purpose of the Study:

  • To evaluate how common dental storage methods affect infrared laser fluorescence.
  • To determine the stability of tooth fluorescence under different storage conditions.
  • To assess the suitability of frozen storage for in vitro fluorescence studies.

Main Methods:

  • Forty extracted human teeth were divided into four groups.
  • Three groups were stored in chloramine, formalin, or thymol solutions at 4°C.

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  • One group was stored frozen (-20°C) without solution, and fluorescence was measured over time.

    • Main Results:

    • Storage in formalin, chloramine, and thymol solutions caused significant fluorescence decreases after 2 years.
    • Frozen storage resulted in a slight, non-significant increase in fluorescence (5%).
    • Solutions significantly influenced fluorescence yield, while frozen storage maintained stability.

    Conclusions:

    • Commonly used wet storage solutions negatively impact infrared laser fluorescence measurements.
    • Frozen storage of teeth is a reliable method for in vitro studies, preserving fluorescence.
    • Fluorescence data from frozen samples can be extrapolated to predict in vivo responses.