Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Precursor B cell receptor signaling activity can be uncoupled from surface expression.

F Betul Guloglu1, Christopher A J Roman

  • 1School of Graduate Studies, Program in Molecular and Cellular Biology, State University of New York-Downstate Medical Center at Brooklyn, NY 11203, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|May 20, 2006
PubMed
Summary

Precursor B-cell receptor (preBCR) signaling can occur from intracellular locations like the trans-Golgi network, not just the cell surface. This finding broadens our understanding of B-cell development and signaling pathways.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Restoring B cell intrinsic tolerance to lupus autoimmunity: a rational strategy for lupus treatment.

Frontiers in immunology·2026
Same author

PKCδ Protects against Lupus Autoimmunity.

Biomedicines·2024
Same author

A MACS protocol for purification of untouched germinal center B cells from unimmunized or germinal center-induced mice.

STAR protocols·2022
Same author

SMS2 deficiency impairs PKCδ-regulated B cell tolerance in the germinal center.

Cell reports·2021
Same author

Immune checkpoint inhibitors in malignancies with mismatch repair deficiency: a review of the state of the current knowledge.

Journal of investigative medicine : the official publication of the American Federation for Clinical Research·2017
Same author

Presentation of high antigen-dose by splenic B220(lo) B cells fosters a feedback loop between T helper type 2 memory and antibody isotype switching.

Immunology·2016

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Precursor B-cell receptor (preBCR) signaling is crucial for progenitor (pro-) B cell proliferation and differentiation into pre-B cells.
  • The cell autonomy of preBCR signaling and low surface expression suggest alternative signaling mechanisms.
  • Investigating intracellular preBCR signaling is key to understanding B-cell development.

Purpose of the Study:

  • To investigate the potential for preBCR signal propagation from intracellular membranes, specifically the endoplasmic reticulum (ER) and trans-Golgi network (TGN).
  • To determine if preBCR complexes retained in intracellular compartments can mediate signaling events.
  • To explore the functional consequences of altered preBCR subcellular localization on B-cell development.

Main Methods:

Related Experiment Videos

  • Engineered preBCRs with modified heavy chains (HCs) to localize to intracellular sites (ER, TGN, lysosomes) using appended localization sequences.
  • Assessed preBCR activity by measuring preBCR-dependent events such as CD2 and CD22 expression and proliferation in transformed and primary pro-B cells.
  • Compared the signaling activity of wild-type preBCRs with intracellularly retained preBCRs.

Main Results:

  • PreBCR complexes retained in the TGN or trafficked to lysosomes showed significant signaling activity (up to 50% of wild-type).
  • This activity occurred despite low or undetectable surface preBCR expression in transformed cells.
  • PreBCRs retained in the ER were inactive in signaling.
  • Intracellular preBCR signaling is tolerant of substantial changes in subcellular distribution within post-ER compartments.

Conclusions:

  • PreBCR signaling can be effectively mediated from intracellular compartments, particularly the TGN.
  • The preBCR can activate signaling pathways from both the plasma membrane and intracellular sites.
  • These findings suggest a more versatile role for the preBCR in regulating B-cell development than previously appreciated.