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An activity-based two-hybrid system for the selective identification of substrates of protein kinases

Jonathan M Shaffer1, Umesh C Karandikar, Ashok P Bidwai

  • 1Department of Biology, West Virginia University, Morgantown, WV 26506, USA.

Analytical Biochemistry
|May 23, 2006
PubMed
Abstract

No abstract available in PubMed .

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Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
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