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Hyaluronate and its receptors in bone marrow
Ulrika M Schade1, Nina Nehmann, Hans-P Horny
1Institute for Anatomy II: Experimental Morphology, University Hospital Hamburg-Eppendorf, Martinistr. 52, D-20246 Hamburg, Germany.
Acta Histochemica
|May 23, 2006
Summary
Cell adhesion involves CD44 and hyaluronate, crucial for cell processes. In bone marrow, CD44 is found on scattered cells, while hyaluronate is limited, suggesting minimal interaction in normal marrow.
Area of Science:
- Cellular Biology
- Immunology
- Hematology
Background:
- Cell-cell and cell-matrix interactions are vital for cellular processes, mediated by cell adhesion molecules like CD44.
- CD44 is a surface receptor interacting with hyaluronate, important in various cells, including the hemopoietic system.
- Limited knowledge exists regarding CD44 and hyaluronate roles within the bone marrow microenvironment.
Purpose of the Study:
- To investigate the expression and localization of CD44 and hyaluronate in human bone marrow.
- To compare CD44 and hyaluronate distribution in situ within bone marrow biopsies versus in cultivated human bone marrow stromal cells.
Main Methods:
- Immunohistochemistry was employed on trephine biopsies of human bone marrow.
- Human bone marrow stromal cells were cultivated and analyzed for CD44 and hyaluronate expression.
Main Results:
- In bone marrow biopsies, hyaluronate deposition was localized to specific extracellular matrix patches, with most areas lacking it.
- CD44 immunopositivity was observed on scattered mast cells and lymphocytes within the bone marrow.
- Cultivated marrow-derived stromal cells showed immunopositivity for CD44, hyaluronate synthase, and hyaluronate.
Conclusions:
- A significant discrepancy exists in CD44 and hyaluronate localization between in situ bone marrow and cultured stromal cells.
- The low number of CD44-positive cells in normal bone marrow suggests limited CD44-hyaluronate interactions in cell adhesion within this tissue.
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