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Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures
Published on: March 28, 2017
Biotransformation of (-)-menthone by human liver microsomes
Mitsuo Miyazawa1, Kyousuke Nakanishi
1Department of Applied Chemistry, Faculty of Science and Engineering, Kinki University, Osaka, Japan. miyazawa@apch.kindai.ac.jp
Abstract:
The aim of the current study was to investigate the metabolism of (-)-menthone by liver microsomes of humans. (-)-Menthone (1) was metabolized to (+)-neomenthol (2) (3-reduction) and 7-hydroxymenthone (3) by human liver microsomes. The metabolites formed were analyzed on GC and GC-MS. Kinetic analysis showed that K(m) and V(max) values for the metabolized (-)-menthone to respective (+)-neomenthol and 7-hydroxymenthone by liver microsomes of human sample HG70 were 0.37 mM and 4.91 nmol/min/mg protein and 0.07 mM and 0.71 nmol/min/mg protein.
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